Univrsity of Tehran Press Iranian Journal of Field Crop Science 2008-4811 44 2 2013 08 23 The Expression of Some Genes Involved in Cold Tolerance in Rice (Oryza sativa L.) Using RT-PCR The Expression of Some Genes Involved in Cold Tolerance in Rice (Oryza sativa L.) Using RT-PCR 227 235 35112 10.22059/ijfcs.2013.35112 FA Khazar EDRISI MARIAN Former Graduate student, Faculty of Agriculture, University of Gilan, Iran Habibollah SAMIZADEH LAHIJI Associate Professor, Faculty of Agriculture, University of Gilan, Iran Mohammad Mehdi SOHANI Assistant Professors, Faculty of Agriculture, University of Gilan, Iran. Seyyed Hasan HASANI Assistant Professors, Faculty of Agriculture, University of Gilan, Iran Journal Article 2012 09 16 Cold stress during the beginning of the season usually causes damage to rice plants in the north of Iran. In this study, the expression of four genes namely OsP5CS2, OsTTP1, OsCOIN and OsLti6a, was studied in 14-day-old seedlings of a cold tolerance PR genotype and a cold sensitive Hashemi variety during cold stress (5°C for 24h) by means of Differential Display method. PCR products were quantified using Total Lab (Ver 1.10) software and significant differences in gene expression were determined using Wilcoxon Test of SPSS.ver.18 software. Different genes displayed diverse expression patterns in tolerant and susceptible plants under cold stress condition. The expression level of OsP5CS2 and OsLti6a genes increased in PR and suppressed significantly in Hashemi genotypes. Two other genes, OsTPP1 and OsCOIN, were significantly up-regulated in PR, but did not have detectable expression in Hashemi. The results suggest that the expression of studied genes changes in response to cold stress and so probably the expression changes of these genes could be applicable in breeding projects in order to develop rice cold tolerance. Cold stress during the beginning of the season usually causes damage to rice plants in the north of Iran. In this study, the expression of four genes namely OsP5CS2, OsTTP1, OsCOIN and OsLti6a, was studied in 14-day-old seedlings of a cold tolerance PR genotype and a cold sensitive Hashemi variety during cold stress (5°C for 24h) by means of Differential Display method. PCR products were quantified using Total Lab (Ver 1.10) software and significant differences in gene expression were determined using Wilcoxon Test of SPSS.ver.18 software. Different genes displayed diverse expression patterns in tolerant and susceptible plants under cold stress condition. The expression level of OsP5CS2 and OsLti6a genes increased in PR and suppressed significantly in Hashemi genotypes. Two other genes, OsTPP1 and OsCOIN, were significantly up-regulated in PR, but did not have detectable expression in Hashemi. The results suggest that the expression of studied genes changes in response to cold stress and so probably the expression changes of these genes could be applicable in breeding projects in order to develop rice cold tolerance.

https://ijfcs.ut.ac.ir/article_35112_c3bbac4058fb05bca780fd14e983ea3b.pdf