Univrsity of Tehran Press Iranian Journal of Field Crop Science 2008-4811 41 3 2010 11 22 Evaluation of cry1Ab Gene in Different Segregating Populations of Rice Evaluation of cry1Ab Gene in Different Segregating Populations of Rice 19581 FA Ghafar Kiani Ghorban Ali Nemat Zadeh Behzad GharehYazi Majid Satari Journal Article 1970 01 01 Through an application of genetic engineering, genes from a soil bacterium Bacillus thuringiensis (Bt) which confer resistance to pests were transferred into rice. Successful employment of genetic engineering in traditional breeding programs is dependent upon stable transmission of transgene in transgenic varieties. Three different segregating F2 populations were developed from a cross between each one of the transgenic lines namely: Tarom Molaii, Neda and Nemat and Sang Tarom while the segregation mode of cry1Ab gene being studied in these populations. The results of phenotypic assay in field and genotypic analysis (employing PCR in laboratory on the different F2 populations) revealed monogenic segregation ratio for cry1Ab gene. The results indicated single copy integration of cry1Ab gene in the genomes of transgenic Tarom molaii, Neda and Nemat. This illustrates predictable segregation pattern of cry1Ab gene in transgenic plants. In addition, the data obtained imply that integration of cry1Ab gene in the genome of the studied transgenic lines is stable and can be successfully transmitted to successive generations. So, these transgenic lines could be well employed for improving pest tolerance of varieties in integrated pest management programs Through an application of genetic engineering, genes from a soil bacterium Bacillus thuringiensis (Bt) which confer resistance to pests were transferred into rice. Successful employment of genetic engineering in traditional breeding programs is dependent upon stable transmission of transgene in transgenic varieties. Three different segregating F2 populations were developed from a cross between each one of the transgenic lines namely: Tarom Molaii, Neda and Nemat and Sang Tarom while the segregation mode of cry1Ab gene being studied in these populations. The results of phenotypic assay in field and genotypic analysis (employing PCR in laboratory on the different F2 populations) revealed monogenic segregation ratio for cry1Ab gene. The results indicated single copy integration of cry1Ab gene in the genomes of transgenic Tarom molaii, Neda and Nemat. This illustrates predictable segregation pattern of cry1Ab gene in transgenic plants. In addition, the data obtained imply that integration of cry1Ab gene in the genome of the studied transgenic lines is stable and can be successfully transmitted to successive generations. So, these transgenic lines could be well employed for improving pest tolerance of varieties in integrated pest management programs https://ijfcs.ut.ac.ir/article_19581_43ba0e5f8732251a916affbad41c6a1a.pdf