@article { author = {Karimzadeh, Ghasem and Ashkani, Sadegh and Ahmadian Tehrani, Parichehreh and Davoudi, Dariush and Mirza Ghaderi, Ghader}, title = {Cytogenetic Studies of some Iranian Wild Wheat Species (Aegilops) and OR Banding}, journal = {Iranian Journal of Field Crop Science}, volume = {41}, number = {2}, pages = {-}, year = {2010}, publisher = {Faculty of Agriculture, University of Tehran}, issn = {2008-4811}, eissn = {2423-8082}, doi = {}, abstract = {In order to deliberate karyologic and cytogenetic parameters, fifteen Aegilops populations, collected from different regions of the country, were studied. These populations included 3 diploids of Ae. tauschii, 6 tetraploids of either Ae. triuncialis or Ae. cylindrica species. The resultant data were analyzed according to a 2-factorial experiment on the basis of a completely randomized design (CRD) with 5 replicates of cells. The factors considered were populations (3 and 12 levels) and chromosome (7 and 14 levels) for diploids and tetraploids, correspondingly. The ANOVA results indicated conspicuous between-diploid differences for L, S, TL and r-value, between-tetraploid differences for RL%, and between-chromosomes either diploid or tetraploid differences for L, S, TL, RL% and F%. Mean comparisons were carried out, using LSR test. Both type populations differed in satellite number, ranging from 0 to 2 pairs. The most chromatin length was detected in pop. 11 (152.18 µm), while pop. 2 bore the least (70.52 µm). Among 15-population karyotypes, 10 were determined in 1A class of Stebbin's classification vs. 5 in 1B class. According to Levan's chromosome nomenclature, the chromosomes of 5 pops. were identified as medium region " m " and those of 10 pops. as both medium region " m " and sub medium region " sm ". Chromosome banding has proven to be a valuable tool for karyotype analysis and for rapid detection of gross chromosomal rearrangements, including large deletion and insertion mutation as well as transpositions. This research was carried out towards an establishment of a procedure, OR-banding, for the detection of the Aegilops chromosomes. Chromosomes OR-banding technique was investigated on 3 Iranian pops. of Ag. tauschii. Chromosome slides were prepared via squash technique in different intervals (24 h to one week) after being slained with 1% aceto-orcein dye. Chromosomal banding pattern was observed 3 d after staining. It was shown that the number of the bands decreased from prophase to metaphase stages of mitosis. The best time for observation of the OR-Banding pattern in Aegilops samples was a long-term (one week) past remaining of the root meristems in dye. This method can be empolyed for an identification of metaphase chromosomes in wild relatives of wheat.}, keywords = {Aegilops,chromosome,Cytogenetics,karyotype,OR Banding,Wild wheat}, title_fa = {مطالعه سیتوژنتیکی برخی از گونه‌های گندم وحشی آجیلوپس ایران و نواربندی OR}, abstract_fa = {به منظور بررسی تنوع سیتوژنتیکی موجود در گونه‌های آجیلوپس، کاریوتیپ 15 جمعیت آجیلوپس بومی مناطق مختلف ایران شامل 3 جمعیت دیپلوئید و 12 جمعیت تتراپلوئید مورد مطالعه قرار گرفت و پارامترهای کروموزومی در هر جمعیت بررسی شدند. نتایج بررسی‌های کاریوتیپی نشان داد که جمعیت‌ها دارای تعداد متفاوتی از کروموزوم‌های ماهواره‌دار بودند. بزرگترین طول کل کروماتین مربوط به جمعیت 11 (2/152 میکرومتر) و کمترین آن مربوط به جمعیت 2 (5/70 میکرومتر) بود. تعداد پنج جمعیت فقط دارای کروموزوم‌های از نوع متاسنتریک (m) و ده جمعیت نیز دارای هردو نوع متاسانتریک و ساب متاسنتریک (sm) بودند. همچنین تقارن کاریوتیپی به روش استبینز، ده جمعیت را در کلاس1A و پنج جمعیت در کلاس 1B قرار داد. تجزیه واریانس پارامترهای کاریوتیپی نشان داد که بین جمعیت‌های دیپلوئید از لحاظ پارامترهای طول بازو‌های بلند و کوتاه، طول کروموزوم و نسبت طول بازوی کوتاه به بلند و در بین جمعیت‌های تتراپلوئید برای اغلب پارامترها اختلاف معنی‌دار وجود داشت. روش نواربندی OR در فواصل مختلف زمانی 24 ساعت تا یک هفته بعد از رنگ‎آمیزی با استو اورسئین 1 درصد، بر روی 3 جمعیت از گونه Ae. tauschii (14x=2n=2) انجام شد. مناسب‌ترین زمان رنگ‌آمیزی برای نواربندی، یک هفته تعیین گردید.}, keywords_fa = {سیتوژنتیک,کاریوتیپ,کروموزوم,گندم وحشی Aegilops,نواربندی OR}, url = {https://ijfcs.ut.ac.ir/article_20985.html}, eprint = {https://ijfcs.ut.ac.ir/article_20985_5fc2eea72396f08771bad0ca8e826da5.pdf} }